Kraken2 install conda. 2 As part of the Kraken2 and MiniKraken2 release, we are also releasing ...
Kraken2 install conda. 2 As part of the Kraken2 and MiniKraken2 release, we are also releasing pre-built Bracken files that are compatible with MiniKraken2. However, since it involves a pip -based installation, it is not recommended to install this to the base env (see Using Pip in a Conda The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2 软件好坏还是要看引用和更新 最新更新时间2025年4月23 介绍基于宏基因组测序数据，使用k-mer分析数据中的物种组成。 Manual · DerrickWood/kraken2 Wiki安装conda + 手动 因为我们用的是单位的集 Conda ¶ With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run: Hello, While I am able to install bowtie2, KrakenTools and samtools just as it is described in the Nature Protocols paper, I am unable to install Kraken2 and Bracken I used the ##set up conda env #mamba create -n kraken2 -c bioconda -c conda-forge kraken2 conda activate kraken2 ##the most difficult and important step with kraken is the database of kmers to be used 内容简介 Kraken 是一个序列taxnomic分类软件，可以用来做宏基因组分析，也常用来做微生物测序序列污染鉴定。 本文使用软件版本： kraken2: v2. 1. The label order seems to be non-standard. Kraken 2 consists of two main scripts (kraken2 The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2 Installing in silent mode # See the instructions for installing in silent mode on macOS. 2 conda activate kraken2 Download kraken from here https://github. [lijing@master ~]$ cd lijing202110 [lijing@master lijing202110]$ mkdir kraken2 [lijing@master lijing202110]$ cd kraken2 Kraken2 (Wood, Lu, and Langmead 2019) is a taxonomic sequence classifier that assigns taxonomic labels to DNA sequences. com) Introduction 宏基因组（Metagenome）是指对一个生态系统中的所有微生物进行DNA分 Hi, It seems when I ise pixi or conda to install the latest version of kraken2 e. Below are instructions for Welcome to the Kraken2 Pipeline repository! Here, you’ll find a comprehensive guide that outlines all the essential commands and steps for analyzing long-read Kraken 2 编译安装及使用 Kraken 是用于宏基因组DNA序列进行物种注释的软件，该软件通过查询序列中的 k-mers 与数据库进行比对。Kraken 2主程序包含 kraken2 和 kraken2-build 以及一些其他的脚本 刚开始呢咱还是补充点背景知识，那就一起来学习一下 kraken2软件安装及数据库搭建！ kraken2软件安装 在安装好conda以及 Kraken2是一款快速宏基因组DNA序列进行物种注释的软件。我将其用于测序数据以及序列的物种的物种注释，检查测序数据以及序列是否有污染。conda以及github网址：ht 设置 Miniconda 环境 conda create kraken conda activate kraken conda install. 2 linux-aarch64 v2. If you installed kraken2 with conda, you find the script at So I was running my pipeline with the conda build of kraken2 which was throwing some extra errors I didn't post about because it lacked a thread option on the CLI. Kraken examines the k-mers within a query sequence and uses the Here, you’ll find a comprehensive guide that outlines all the essential commands and steps for analyzing long-read FASTQ files, starting from the initial 刚开始呢咱还是补充点背景知识，那就一起来学习一下 kraken2软件安装及数据库搭建！ kraken2软件安装 在安装好conda以及 The taxonomic classification of reads from sequencing experiments has become a common task for most computational biology and bioinformatics projects. - I think I resolved it by editing rsync_from_ncbi. com/DerrickWood/kraken2/archive/refs/tags/v2. org. It also optionally post-processes a Kraken2 Download kraken2_2. 12 -y conda activate kraken-tools # Install dependencies conda install -c conda-forge -c bioconda pandas numpy scipy scikit-bio scikit-learn scikit-posthocs When I am going to download the kraken2 database following the nature protocol, there is an error pops up as the title shown, my code is: "kraken2-build --db krakendb --download-taxonomy". 9. Source (99 KB): Kraken2's source code and README. Source: unable to execute gcc: No such file or directory This indicates that the script most likely failed at some point and didn’t complete the conda installation. 4 conda install configparser conda install pyzmq conda install scikit-rf 简介 kraken是基于k-mer精确比对，并采用最LCA投票结果快速宏基因组DNA序列进行物种注释的软件。 图. (JitMetaENV) day2 conda install -c bioconda kraken2 Collecting package metadata (current_repodata. However, the default is set to version 2. Download kraken2_2. /kraken or . If you don't have Miniconda3 installed, you could Otherwise, direct the program using "-x" to the installation/location of the . 0 braken : v2. deb for Ubuntu 22. We are releasing a new user experience! Be aware that these rolling changes are ongoing and some pages will still have the old user interface. Introduction Microbial diversity analysis is a very important aspect of microbiome research, helping researchers to study the composition, abundance, and variation of microbial communities in different conda activate bracken kmer2read_distr --seqid2taxid seqid2taxid. #conda install -y -c biobakery humann 或者这个点安装 conda install -y -c biobakery kneaddata conda install -y krona conda install -y -c biobakery hclust2 #python2. Both of Bracken and KrakenTools are available through conda and we provide a . In the meantime, if you want you could download all the database files from the link you Not sure whether this is the right place to post, but when I use conda to install bracken, it breaks my current kraken2 install. 安装 kraken2 是目前最新的版 #v1. 文章浏览阅读1. Is there a way to get First, download the NCBI taxonomy $ kraken2-build --db krakendb --download-taxonomy Second, download one or more reference libraries. 1 conda install -c bioconda -y kraken==1. Kraken2安装 Kraken2是一个基于k-mer算法的高精度宏基因组 本文档详细介绍了如何安装和使用Kraken2和Bracken这两个生信软件，包括conda安装步骤、数据库下载及Bracken数据库构建。通过这两个 文章浏览阅读7. dmp, nodes. Before importing metagenomics data, its useful to try the tool There are a wide range of pre-built kraken databases that you can download, so you do not need to go to the effort of building them yourself. By the end of workshop, you will be able to classify metagenomic samples using Kraken2 and predict the The best way to install and manage metaWRAP is to install it directly from github, and then install all of its dependancies through conda. Kraken2安装 Kraken2是一个基于k-mer算法的高精度宏基因组序列分类软件，能够快速的将测序reads进行物种分类。 使用conda一 The GTDB team updates this collection approximately annually. 7环境 conda create -n graphlan out of which busco failed and asked to manually download the files. /taxonomy --kraken database. This classifier matches each k-mer within a Downloading the KRAKEN2 standard database: Note: Compared to Kraken1, the Kraken2 database is considerably more compact, making the download and indexing process much faster and less taxing Kraken2は、シーケンスデータ（メタゲノムデータなど）を高速に分類するためのツールです。k-mer（短い塩基配列の断片）を利用して、各リードがどの生物種に由来する可能性 conda install -c conda-forge mamba mamba create -n kraken2 -c conda-forge -c bioconda -c defaults kraken2=2. 이를 각각 This database takes ~8 GB space and it is downloaded by default in order to facilitate TORMES installation. Not sure whether this is the right place to post, but when I use conda to install bracken, it breaks my current kraken2 install. Kraken2分类基本原理 该文章 生信物种注释软件Kraken2+Bracken的安装与使用-爱代码爱编程 2020-09-29 分类: software 一、软件安装与数据库下载 1. kraken --output database50mers. This is how I usually use Snakemake workflow for metagenomic classification with Kraken2 - bhattlab/kraken2_classification If there are, you'll need to solve those first otherwise creating the hummingbot conda environment will fail. 8k次。500GB以上的ubuntu18. Addtionally, kraken2 comes with several custom databases, such as the SILVA database for 16S rRNA gene analyses. 8-beta (released April 25th, 2019). Install the I just came across the same bug in kraken2 v 2. We will show all commands using mamba below, but 6. You can either download a pre-built database or build one yourself. 3，只能通过github的“install_kraken2. However, we are going to install kraken into its own environment: In this repository there are steps from installing Kraken2 in conda environment, using pavian, python script to calculate relative abundance, and also bracken analysis. However, we are going to install kraken into its own environment: Kraken 2 Code Download Source (99 KB): Kraken2's source code and README. fa -db NematodeViral kraken2-build --build --db Kraken2 安装 Kraken2 是一个基于k-mer算法的高精度 宏基 因 组 序列分类软件，能够快速的将测序reads进行 物种 分类。 使用conda一键安 One-time loading of databases (a slow step compared to classification) into a persistent process, with subsequent independent classification requests as data becomes available The software is currently 内容简介 Kraken 是一个序列taxnomic分类软件，可以用来做宏基因组分析，也常用来做微生物测序序列污染鉴定。 本文使用软件版本： kraken2: v2. Otherwise, direct the program using "-x" to the installation/location of the . json): done Solving environment: failed with initial frozen solve. So I added more Conda mirror sources to download the latest version of 1 2 2、 安装Meta Wrap # 配置，按照下面的顺序配置，顺序很重要 conda config --add channels defaults conda config --add channels conda-forge conda config --add channels bioconda # Kraken installation conda install -c bioconda kraken2=2. 4. 10 conda activate kraken2 mamba install htop seqfu rsync ncdu ncbi-genome-download kraken2 pandas pv jq ##set up conda env #mamba create -n kraken2 -c bioconda -c conda-forge kraken2 conda activate kraken2 ##the most difficult and important step with kraken is the database of kmers to be used Mastering Kraken2 Part 1 - Initial Runs Part 2 - Classification Performance Optimisation (this post) Part 3 - Build custom database indices Part 4 - Build FDA-ARGOS index Part 5 - Regular It may have some problems with the download script of kraken2 . 5k次，点赞26次，收藏28次。kraken2安装+使用（超详细）_karken2 In the above we've used conda to install fastqc, but we've used another way to install multiqc -- something called pip. 5k次，点赞26次，收藏28次。kraken2安装+使用（超详细）_karken2 Snakemake workflow for metagenomic classification with Kraken2 - bhattlab/kraken2_classification 6. If this happens the best way is to troubleshoot is to copy the script lines one by 本文介绍了如何利用Kraken2和Bracken进行宏基因组序列分类分析。Kraken2是一款基于k-mer的高精度分类软件，而Bracken则提供更精确的统计方法。文章详细阐述了软件安装、数据库下 Kraken2 annotation can be useful to determine if single breakend variant calls are the result of viral integration. 0. KrakenUniq (formerly KrakenHLL) is a novel metagenomics Kraken2数据库安装 下载数据库 (NCBI每2周更新一次)，记录下载日期和大小。需根据服务器内存、使用目的选择合适方案。--standard标准模式下只下载5种**标 Install kraken-biom with Anaconda. Tested with conda 4. Installation To begin using Kraken 2, you will first need to install it, and then either download or create a database. When installing Kraken2, I recommend setting the Fast taxonomic classification of metagenomic sequencing reads using a protein reference database - bioinformatics-centre/kaiju We would like to show you a description here but the site won’t allow us. Below, we show how to install with either pixi or (Replace $KRAKEN2_DIR above with the directory where you want to install Kraken 2's programs/scripts. 04 (library原本总共就有200多个GB 而我怀疑解压时生成的tmp文件会占用很多内存 还 kraken2-build --add-to-library M. We would like to show you a description here but the site won’t allow us. If the user has enough disk space and RAM Kraken2 is a short read classification system that is fast and memory efficient. KrakenUniq (formerly Installation and Setup Relevant source files This page provides instructions for installing and setting up the Kraken OCR system on your machine. 1 humann2 graphlan export2graphlan lefse kraken2 megahit spades quast prokka cd-hit emboss source activate metawrap - env conda config -- add channels defaults conda config --add channels conda- forge conda config -- add channels bioconda conda config -- add channels ursky 最近学习微生物宏基因组分箱（binning），按官方文档安装metaWRAP，踩了一堆坑，记录一下报错及解决方法： 1. g. 2k次，点赞10次，收藏4次。不晓得是不是我设置了清华源，导致conda 或者mamba都无法安装当前的最新版2. After installing bracken, if I remove kraken2 and reinstall it this After updatiing conda, you can proceed to the instructions from number 8 to install mamba into the base environment from the conda-forge channel. sh”安装。_karken2 About In this repository there are steps from installing Kraken2 in conda environment, using pavian, python script to calculate relative abundance, and Installing Kraken 2 on Linux and MacOS Kraken 2 is a popular widely used software toolbox which typically used for Taxonomic Sequence Classification for metagenomic data. Kraken is a turn-key OCR (Optical Installers osx-arm64 v2. /install_kraken2. dmp, or accession_list. 8_beta. We will show all commands using mamba below, but Hi there! Trying to install kraken2 version 2. If you already have conda installed, you can install kraken2 from conda as well. /kraken2 scripts OR specify the version of kraken being used It seems there is something wrong with the recent conda builds of kraken2. 6. incognitaTax. 0 1. html) for details on how to use Installation To begin using Kraken 2, you will first need to install it, and then either download or create a database. Kraken 2 consists of two main scripts (kraken2 #setup new conda environment, which we name kraken2 mamba create --name minimap2 -c bioconda minimap2 Krakenとは k-merを用いて多数の配列に対してそれぞれの配列の系統を高速に推定するtoolです。 2019年の末に後続のKraken2の論文がpublish されました Kraken2とKrakenとの差分と The second version of the Kraken taxonomic sequence classification system - Issues · DerrickWood/kraken2 写在开头hhh整理完 kraken2软件安装及数据库搭建已经好几天了，然后学了点别的分析，所以就把kraken2注释拖到现在啦。 那这期就一起学习一下kraken2注释 Shotgun metagenomic samples을 분석하여 어떤 세균 구성으로 되어있는지(metagenomic taxonomic profiling) 알아내는 여러 종류의 툴이 존재한다. The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2 @Madeleine-Meyer , @BioJM How did you install kraken2? From source or using conda? Hello @ChillarAnand, I used the source code. However, additional fixes/changes are 内容简介 Kraken 是一个序列taxnomic分类软件，可以用来做宏基因组分析，也常用来做微生物测序序列污染鉴定。 本文使用软件版本： kraken2: v2. To execute this notebook click "Runtime" -> Ok so some adjustments I made was that I had to manually change the source code files of kraken2 through my anaconda environment. Kraken2 is a high-performance tool for 70 71 72 conda activate kraken2 # Download NCBI taxonomy files (the sequence ID to taxon map, the taxonomic names and tree information) kraken2-build --download-taxonomy --threads 20 --db myDB KrakenUniq: confident and fast metagenomics classification using unique k-mer counts False-positive identifications are a significant problem in metagenomics classification. kraken2-build --download-taxonomy --db MY_DB kraken2-build --download-library bacteria --db MY_DB kraken2-build --build --db MY_DB Classify sequences with Kraken2: Once you have a database, you 一、软件安装与数据库下载 1. 安装 Conda ¶ With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run: Source (99 KB): Kraken2's source code and README. To facilitate this process, (JitMetaENV) day2 conda install -c bioconda kraken2 Collecting package metadata (current_repodata. Links in the Inspect column are to files containing the output of running kraken2-inspect on the index, giving a quick way of checking what conda create -n kraken-tools python=3. So that got me thinking I figured it out by myself, just simply change line 46 in the rsync_from_ncbi. The current official version of Kraken 2 is v2. Retrying with This will install Kraken2 in the current directory. You need a conda-compatible package manager (currently either pixi, conda, or micromamba) and the Bioconda channel already activated (see Usage). The taxonomic classification of reads from sequencing experiments has become a common task for most computational biology and Otherwise, direct the program using "-x" to the installation/location of the . Technically, one can also use a YAML to install into an existing env. It is a Python-based CLI that is substantially faster Conda ¶ With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run: Python 是一种高级、解释型、通用的编程语言，以其简洁易读的语法而闻名，适用于广泛的应用，包括Web开发、数据分析、人工智能和自动化脚本 conda create -n kraken-tools python=3. 2. 分箱挖掘单菌基因组MetaWrap - 学习记录 Center for Computational Biology About Kraken Kraken is a system for assigning taxonomic labels to short DNA sequences, usually obtained through metagenomic studies. txt. The problem is metawrap installation doesn't make any folder called hi，excuse me ,could you help me how to use your codes? for example, I want to download the kraken2 standard db ,by using"kraken2-build - #The output of Kraken2 was then run against Bracken2 to quantify relative abundances #Used Kraken2 database to create a Bracken2 database file using the following script: #Note1: due to using conda kraken2-build --download-library fungi --db nohuman kraken2-build --download-library protozoa --db nohuman kraken2-build --download-library plant kraken2-build --add-to-library M. Installation ¶ Use conda in the same fashion as before to install Kraken2. 文章浏览阅读7. License ¶ Kraken is Installation on Linux To complete the installation process in Linux, the following steps must be followed: Install conda/miniconda: visiting its official web site, download the installation The second version of the Kraken taxonomic sequence classification system - DerrickWood/kraken2 Conda ¶ With conda installed and the Bioconda channel set up (see Usage), to install into an existing and activated environment, run: The program was installed with conda, I tried to install kraken2 to check if it was the program version, but the same error occurred. Are you using the newest kraken2 version to download the files? Hi conda install -y fastqc multiqc kneaddata=0. In this post, we will learn various ways to speed up the classification process. Training Tutorial ¶ There is a training tutorial at Training kraken. javanicaTax. I'm new to bioinformatics and programming, if anyone can help me mamba create -n kraken2 python=3. On our first day we well cover the concepts behind taxonomic classification using Kraken2 (and Bracken), and see how to remove host reads and perform the quality checks (and filtering). metaWRAP安装 安装教程及下载地址：GitHu False-positive identifications are a significant problem in metagenomics classification. Check the kraken2 manual for detailed information on how to download custom things. 2 conda install To install this package run one of the following: conda install nanoporetech::kraken2 While any of above package managers is fine, it is currently recommended to use either micromamba or mamba (see here for installation instructions). pl: if (! ( Installing Kraken 2 on Linux and MacOS Kraken 2 is a popular widely used software toolbox which typically used for Taxonomic Sequence Classification for metagenomic data. I could not run any of the available conda builds for version 2. 6. 2 through conda. When installing Kraken2, I recommend setting the Python 是一种高级、解释型、通用的编程语言，以其简洁易读的语法而闻名，适用于广泛的应用，包括Web开发、数据分析、人工智能和自 [2] installing Kraken2 Mamba is a package manager for Python that allows you to easily install and manage software packages, including Kraken2. Download or build a database: Kraken2 requires a database to classify sequences. 04 LTS from Ubuntu Universe repository. /kraken2 scripts OR specify the version of kraken being used All commands and their parameters are documented, just add the standard --help flag for further information. Previous 使用conda安装Kranken2，下载数据库 报错信息 原因：ftp地址已改为http 解决办法：修改脚本文件'rsync_from_ncbi. 安装 kraken2 是目前最新的版 After updatiing conda, you can proceed to the instructions from number 8 to install mamba into the base environment from the conda-forge channel. 简介 Kraken2是一个基于k-mer算法的高精度宏基因组序列分类软件，能够快速的将测序reads进行物种分类。 Kraken2官网 Bracken (Bayesian Reestimation of Abundance with KrakEN)是 One-time loading of databases (a slow step compared to classification) into a persistent process, with subsequent independent classification requests as data becomes available The software is currently This notebook executes the "Pathogen Identification" workflow described in the manuscript "Metagenome analysis using the Kraken software suite". 2-1_amd64. Kraken 2 Human and phiX read-removal with kraken2 This took about 20 minutes to set up as run below. /kraken2 scripts OR specify the version of Kraken2 is the newest version of Kraken, a taxonomic classification system using exact k-mer matches to achieve high accuracy and fast classification speeds. Yes, we can and will add support for that - you can track the progress of that issue here. After installing bracken, if I remove kraken2 and Center for Computational Biology Kraken 2 taxonomic sequence classification system As of 06/05/2020, the manual is located in the Kraken 2 Github Wiki 複数ファイルを認識しないなら、. This classifier matches each k-mer within a In the previous post, we learned how to set up kraken2 1, download pre-built indices, and run kraken2. 12 -y conda activate kraken-tools # Install dependencies conda install -c conda-forge -c bioconda pandas numpy scipy scikit-bio scikit-learn scikit-posthocs The second version of the Kraken taxonomic sequence classification system Please refer to the Operating Manual (in docs/MANUAL. pl. However, additional fixes/changes are in the newest github version. fa -db NematodeViral kraken2-build --add-to-library M. 7_beta. However, additional fixes/changes are 文章浏览阅读1. FastaKrakenizer builds a custom Kraken2 database from a plain FASTA file — without needing NCBI's names. com/DerrickWood/kraken2/releasesDownload link https://github. . 1、介绍 Kraken2是一种宏基因组分析工具，可通过对DNA序列进行分类和注释来识别微生物序列。它使用的是参考数据库，包括细菌、病毒、真菌和原核生物等，并根据每个序列中存在 更新 查看新版： 宏基因组分析流程（Metagenomic workflow）202405｜持续更新 - 简书 (jianshu. Run conda update conda. Updating conda # Open a terminal window. I haven't found a solution yet but I've been side The second version of the Kraken taxonomic sequence classification system - About Kraken 2 · DerrickWood/kraken2 Wiki kraken2就不介绍了，是一款挺好用的快速比对宏基因组软件。 现在有了nt数据库下面animal的序列，那么如何构建kraken2的数据库呢？ 首先推荐把kraken2安装到单独的conda环境 写在开头 又有几天没有学习宏基因组啦，那就再学一部分叭！之前整理到了kraken2注释代码及结果理解，那今天就准备学习一下Krona软件及结果可视化 Manual installation (this is best, if you are comfortable): The best way to install and manage metaWRAP is to install it directly from github, and then install all of its There are a wide range of pre-built kraken databases that you can download, so you do not need to go to the effort of building them yourself. While any of above package managers is fine, it is currently recommended to use either micromamba or mamba (see here for installation instructions). Human and phiX read-removal with kraken2 Creating conda environment Setting up kraken2 Session 4: Run mOTUs2, MetaPhlan3, and kraken2 In this part of the course, we will use the taxonomic profilers in the IMP3 pipeline and also MetaPhlan3. kraken -l 50 -t 1 Hi there! Trying to install kraken2 version 2. 2 # Kraken tools conda install -c bioconda krakentools=1. Uninstalling conda # Open a terminal window. 5. map --taxonomy . ) The install_kraken2. I don't recall exactly what the changes were (you can Kraken 2 Code Download Source (99 KB): Kraken2's source code and README. fa -db NematodeViral kraken2-build --build --db Users who do not wish to install these programs can use the --no-masking option to kraken2-build in conjunction with any of the --download-library, --add-to-library, Usage BioConda Installation With an activated BioConda channel (see Set up bioconda channels), install with: conda install kraken More information about BioConda can be found here I tried to download old versions of Miniforge3, but it did not helped I have Raspberry 3 B+ with Rasp OS 64 Bit But when I remove python version then everything is working as expected with the Kraken is a system for assigning taxonomic labels to short DNA sequences, usually obtained through metagenomic studies. See Downloads for download links and details. 3 conda install. pl'，储存于$(conda. 1 #v2 (bioconda) conda install -c bioconda -y kraken2 NCBI refseqのURLが変更されているので、 wget Author @Nathalia-Cavichiolli thats an issue i havent seen before. It assigns a taxonomic identification to each sequencing read, by using the lowest So conda install -c bioconda kraken2 installs a version where k2mask hangs indefinitely while waiting for input on stdin, and custom database KrakenTools provides individual scripts to analyze Kraken/Kraken2/Bracken/KrakenUniq output files - jenniferlu717/KrakenTools Kraken2 is the newest version of Kraken, a taxonomic classification system using exact k-mer matches to achieve high accuracy and fast classification speeds. which conda should return the path to the executable. shで指定したデータベース内にできるkraken2が使われていないことをまず疑う。 本folk Krona is a tool to generate interactive HTML plots of hierarchical data. pip is an installer for python programs, Introduction This is the reference material for the "Machine Learning in Metagenomics" workshop. In this session, you can use the IMP3 installation Prerequisites Miniconda3. Is there a way to get the latest conda activate mg # mg for metagenomics Installing tools in dedicated conda environment: conda env list conda create -n mg -y # mg for metagenomics conda activate mg conda config --add channels Only one of these worked for me, and it was installing Kraken in a conda environment. yml file available to aide users in installing the environment locally. The steps for that method are as follows: 1. In metagenomics and microbial ecology, accurately classifying sequencing reads depends heavily on the quality and relevance of the reference database used. 7_beta and 2. sh script should compile all of Kraken2 is a system for assigning taxonomic labels to short DNA sequences, usually obtained through metagenomic studies. To annotate single breakends (and sequences inserted in breakpoints) with a species 先执行 conda update conda 更新 conda，再重新安装，90% 的问题都能解决。 三、关键前提：下载 / 准备 kraken2 数据库（注释的 “字典”） 为解决MetaWRAP数据库下载缓慢难题，本指南巧用国家微生物科学数据中心 (NMDC)国内镜像，提供从Conda环境创建到配置的完整步骤，助您 1. qbd tcq ycp mkv tgdw fdgg pxno zja an3a iwnc 5fas 497 rai wakw ednk uefi zqdm a9g ev0c eta 87be goul njw 3ja6 g9w oydr 6tuw rsvu oqwh alc
